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Accordingly, production of the endogenous antioxidant glutathione (GSH) by glial cells within the brain [8], particularly astrocytes [8,9,10], is a critical function, as most antioxidants are unable to cross the blood brain barrier

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After incubation at 37 °C for 1 h, the media were removed and formazan crystals in the cells were dissolved in 1 mL DMSO and processed for OD reading at 570 nm using a spectrophotometer

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