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Finally, there are other neurotrophic factors that have shown dopaminergical neuronal protection in Parkinson-like models, including hepatocyte growth factor (HGF), mesencephalic astrocyte-derived neurotrophic factor (MANF), cerebral dopaminergic neurotrophic factor (CDNF), granulocyte colony-stimulating factor (G-CSF), and platelet derived growth factor (PDGF-CC) [31-32, 135-136]
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Virus challenge and mouse therapy studies Determination of the viral titer required to kill 50% of inoculated mice (MLD 50 ) was achieved by anesthetizing 6–9 week old BALB/c mice (5 mice/group) with isoflurane and inoculating the mice intranasally (IN) with 50 µl of serial 10-fold dilutions of influenza virus
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