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Quantitative real-time polymerase chain reaction Total RNA (1 µg) was extracted from IPEC-J2 cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) and used for cDNAs synthesis with a Maxima First-strand cDNA Synthesis Kit (Life Technologies)

tone brightening

Typology:2in1

Lowering oxygen levels to 5%, 1% and 0.1% for 6 h before reoxygenation to ambient levels confirmed rapid and reversible NAD reduction in Arabidopsis leaf tissue at 1 and 0.1% oxygen (Fig